NOVEL LUMINESCENT PROBE BASED ON A TERBIUM(III) COMPLEX FOR HEMOGLOBIN DETERMINATION

摘要

We have studied the spectral luminescent properties of Tb(III) and Eu(III) complexes with a number of novel derivatives of oxoquinoline-3-carboxylic acid amides (L_1-L_5). We have observed quenching of the luminescence of 1:1 Tb(III)-L_(1-5) complexes by hemoglobin (Hb), which is explained by resonance energy transfer of electronic excitation from the donor (Tb(III)-L_(1-5)) to the acceptor (Hb). Using the novel luminescent probe Tb(III)-L_1, we have developed a method for determining Hb in human blood. The calibration Stern-Volmer plot is linear in the Hb concentration range 0.6-36.0 μg/mL, detection limit 0.2 μg/mL (3·10~(-9) mol/L).