Rapid Detection and Characterization of Foodborne Pathogens by Molecular Techniques

摘要

Penned by a distinguished and internationally respected authority on food safety, the book provides a detailed assessment and summary of hundreds of contemporary studies focusing on the molecular detection, quantification, and subspecies differentiation of pathogens isolated from foods. The author concentrates on the genes linked to bacterial pathogenicity that have been used for Polymerase Chain Reaction (PCR) detection and identification including discussions of extractions from complex food matrices and DNA purification techniques. The review includes method limitations for certain pathogens or food matrices prefaced by a comprehensive assessment of real-time PCR, including theoretical and operational concepts. A separate chapter is dedicated to each reviewed pathogen.