POROUS POLYDIMETHYLSILOXANE MEMBRANES FOR ENZYME IMMOBILIZATION

摘要

Porous polydimethylsiloxane (PDMS) membranes with alpha-amylase or glucose oxidase activity were prepared by catalytic hydrosilylation cure of PDMS in the presence of the enzyme-water solution. The pores in the membrane are the result of hydrogen foams, which are generated during the curing reaction. The enzyme reactions were examined in batch and permeation experiments by using glucose and starch solutions as substrates. For the permeation set-up, the reaction yields of the immobilized alpha-amylase increased as the permeation rate of the starch solution decreased. The Michaelis-Menten type of reaction kinetics for the immobilized enzyme indicated that the permeation system is effective for the diffusion through the solute of the matrix, as compared with the batch system. (C) 1996 John Wiley & Sons, Inc. [References: 24]