Development of a capillary zone electrophoresis method for determination of mebendazole and levamisole hydrochloride in a combined tablet and a comparison with a LC method.

摘要

The separation and determination of mebendazole (MEB) and levamisole hydrochloride (LH) from a combination tablet by capillary zone electrophoresis (CZE) was developed and validated. The separation was performed in a 60 cm (50.5 cm to detection window) x75 micro m internal diameter fused silica capillary using a background electrolyte of NaH₂PO₄ (20 mM/L, pH 3.0) at 25 kV. Good separation was obtained in less than 8 min. The LOQ values for LH and MEB were 1.43 and 2.50 micro g/mL, respectively; calibration curves were linear from 10 to 500 micro g/mL with r2 >0.999. Mean recoveries of the analytes were greater than 96%. In addition, a comparison with the LC method described in the 2010 Chinese Pharmacopoeia demonstrated that the developed CZE method was equally comparable with regard to linearity, sensitivity, precision, and accuracy, but the analysis time and reagent consumption were decreased. The CZE method is an effective and lower-cost alternative to the LC method.