Preparation and Characterization of Chitosan/ p-cyclodextrin Nanoparticles Containing Plasmid DNA Encoding lnterleukin-12

摘要

Interleukin-12 (IL-12) as a cytokine has been proved to possess antitumor effects via stimulating the immune system. Non-viral gene delivery systems offer several advantages, including easiness in production, low cost, safety; low immunogenicity and can carry higher amounts of genetic material without limitation on their sizes.Methods: pUMVC3-hIL12 loaded Low Molecular Weight chitosan/p-cyclodextrin (LMW CS/ CD) nanoparticles were prepared using iono-tropic gelation method and characterized in terms of size, zeta potential, polydispersity index, morphology, loading efficiency and cytotoxicity against the CT-26 colon carcinoma cell line.