Chloride sensitive probes for biological applications

摘要

Three fluorescent probes have been produced by the quaternisation of 6-methylquinoline with methyl bromide,methyl iodide and 3-bromo-1-propanol.The probes are water-soluble and their fluorescence is quenched by modest concenrtations of chloride ions.One of the dyes,dye 1,has a chloride Stern-Volmer constant of 255 mol~(-1) dm~3,which is one of the highest teported.Fluorescence quantum yields of the dyes have been determined using quinine sulphate as a reference and are all found to be similar lying in the range 0.5-0.64.Applications for chloride determination in biological systems using these probes are discussed.