A study of the binding of C.I.Mordant Red 3 with bovine serum albumin using fluorescence spectroscopy

摘要

The interaction of C.I.Mordant Red 3 and bovine serum albumin was investigated using fluorescence and UV-vis absorption spectroscopy.Fluorescence quenching,from which binding parameters were evaluated,revealed that the quenching of the serum by C.I.Mordant Red 3 resulted from the formation of a dye-serum complex.The enthalpy and entropy changes for the reaction were-50.49 kJ mol~(-1)and-50.88 J mol~(-1)K~(-1)respectively,van der Waals forces and hydrogen bonds were the dominant inter-molecular forces that stabilize the complex;the distance between donor and acceptor was 2.79 nm,according to Forster's non-radiative energy transfer theory.The effect of the dye upon the conformation of bovine serum albumin was analyzed using synchronous fluorescence spectrum.