Insight into the Heparin-Toluidine Blue(CI.Basic Blue 17)interaction

摘要

Heparin-Toluidine Blue(C.I.Basic Blue 17)interaction was studied using UV-vis spectroscopic analysis of the titration of the functional groups present on the polyanion chain.The study took into consideration both the pH and relative amount of the dye as a function of the Heparin repeat unit number;formation of a dye/polysaccharide aggregate was studied under the same conditions and structural models of the two types of aggregates were proposed.As a consequence of such an investigation a new Heparin determination methodology was developed.