Adipokine Gene Single-Nucleotide Polymorphisms in Portuguese Obese Adolescents:Associations with Plasma Concentrations of Adiponectin, Resistin, IL-6, IL-1 beta, and TNF-alpha

摘要

Background: The genetic contribution to obesity and to circulating adipokine levels has not been completely clarified. We aimed to evaluate adipokine genes' single-nucleotide polymorphism (SNP) prevalence and its association with circulating adipokine levels and risk factors for cardiovascular disease in an obese Portuguese pediatric population.Methods: Two hundred forty-eight obese adolescents (mean age 13.4 years old; 47.2% females) participated in a cohort study. We screened 12 SNPs by direct sequencing in five adipokine genes: adiponectin (ADIPOQ: rsl6861194, rsl7300539, rs266129, rs2241766, ral501299), interleukin-1 beta (IL-1beta rsl 143627), IL-6 (IL-6; ral800795), tumor necrosis factor-a (77VF-a; rsl800629), and resistin (RETN;rs1862513, ra3219177, rs3745367, ra3745368). Biochemical analysis included determination of circulating adipokines, C-reactive protein (CRP) levels, lipid profile, and markers of insulin resistance.Results: Compared to males, females presented higher circulating levels of insulin, adiponectin, IL-6, resistin, and leptin concentrations, but lower TNF-a levels. No statistically significant differences were found for genotype or allelic distributions between genders. In the whole sample population, adiponectin levels were influenced by ADIPOQ ral7300539 (c.-1138G>A; lower in subjects with GG genotype). When only males were considered, IL-1/5, IL-6, and TNF-a levels were associated with ADIPOQ rol501299 (c,214 + 62G>T; higher in GG subjects). TNF-a concentrations were modulated by TNF-a rsl800629 (c.-488G>A; lower in GG males), RETNrs 1862513 (c.-216C>G; higher in CC subjects), and RETN rs32l9lll (c.H8 + 39C>T; higher in CC subjects). Leptin levels were influenced by IL-1 beta rsl 143627 (c.-118C>T) presenting TT individuals' lower levels.Conclusions: Our data demonstrate that in pediatric obese patients, some adipokine gene SNPs have an association with circulating adipokine levels and lipid profile.