Repeated measurements of 11beta-HSD-1 activity in subcutaneous adipose tissue from lean, abdominally obese, and type 2 diabetes subjects--no change following a mixed meal.

摘要

The aim of this study was to measure 11beta-HSD-1 activity in subcutaneous adipose tissue by an ex vivo method in three subgroups; lean, obese, and type 2 diabetes subjects, both in the fasting state and after a mixed meal and to determine the variability and reproducibility of this method. Eighteen subjects were investigated; 6 lean, 6 abdominally obese, and 6 type 2 diabetes subjects (BMI 22 +/- 1, 30 +/- 3 and 31 +/- 3 kg/m(2), respectively). Needle biopsies were taken repeatedly and an index of 11beta-HSD-1 activity was measured as percent conversion of (3)H-cortisone to (3)H-cortisol/100 mg tissue. For two separate biopsies taken in the fasting state on the same day, the within subjects CV was 16% and the between CV was 36% for 11beta-HSD-1 activity for all subjects. For two biopsies taken in the fasting state at two different days, the total within subjects CV was 38% and the between subjects CV was 46%. Lean subjects had lower 11beta-HSD-1 activity (4.8 +/- 1.5% conversion of (3)H-cortisone to (3)H-cortisol/100 mg tissue) than both obese (14.4 +/- 1.6% conversion, p<0.01) and type 2 diabetes subjects (11.7 +/- 1.9% conversion, p<0.05) in the fasting state. There was no effect of a meal on 11beta-HSD-1 activity in any of the three groups. The conclusions from this study are: 1) the variation coefficient for the ex vivo adipose tissue 11beta-HSD-1 activity method was approximately 25% for repeat measures within subjects; 2) food intake had no major impact on enzyme activity; and 3) 11beta-HSD-1 activity in subcutaneous adipose tissue was significantly increased in obese subjects with or without T2DM compared to lean subjects without diabetes.