One-cell biopsy significantly improves the outcome of preimplantation genetic diagnosis (PGD) treatment: Retrospective analysis of 569 PGD cycles at the Stockholm PGD centre

摘要

STUDY QUESTION What are the significant factors that influence the outcome of a PGD treatment? SUMMARY ANSWER The age of the woman and the number of biopsied cells per embryo are of significant importance for a successful PGD treatment. WHAT IS KNOWN ALREADY Younger women are more likely to succeed with an IVF treatment. STUDY DESIGN , SIZE, DURATION Cohort study, retrospective analysis of 569 PGD cycles, 19962009. PARTICIPANTS, SETTING , METHODS 256 couples and 569 PGD treatments at 'Stockholm PGD centre'. At this centre after 2003, a 1-cell policy was applied, when possible, with respect to the reliability of the diagnostic test and since 2009, 1-cell biopsy policy was also applied for monogenic disorders. MAIN RESULTS AND THE ROLE OF CHANCE The women under 36 years of age were three times more likely to get pregnant after PGD treatment, P 0.003 and odds ratio 3.1 [95 confidence interval (CI) 1.56.5]. The 1-cell biopsy cycles were twice as likely to result in a pregnancy in comparison with cycles were 2 cells were removed from the embryo, P 0.0013 and odds ratio 2.55 (95 CI 1.444.52). No other factors were found to be significant for the outcome. LIMITATIONS, REASONS FOR CAUTION Retrospective analysis with 1- and 2-cell biopsies at different times. WIDER IMPLICATIONS OF THE FINDINGS The results will have an impact on the implementation of PGD in general, thereby making it possible to significantly improve the treatment outcome.