A two-step procedure for adventitious shoot regeneration from in vitro-derived lingonberry leaves: shoot induction with TDZ and shoot elongation using zeatin.

摘要

The effects of TDZ (0, 0.1, 1, 5 and 10 micro M) and explant orientation on adventitious shoot regeneration of 'Erntedank' lingonberry (Vaccinium vitis-idaea) were studied. Moderate concentrations (1 to 5 micro M) of TDZ supported bud and shoot regeneration, but strongly inhibited shoot elongation. TDZ-initiated cultures were transferred to medium containing 1-2 micro M zeatin and produced usable shoots after one additional subculture. Adventitious bud and shoot regeneration was greatly influenced by explant orientation. Elongated shoots were rooted on a 2 peat:1 perlite (v/v) medium, and the plantlets were acclimatized and eventually established in the greenhouse with 80-90% survival rate.