Molecular cloning of the murine branched chain a-ketoacid dehydrogenase E2 subunit: presence of 3' B1 repeat elements

摘要

The cDNA sequence encoding the murine E2 subunit (dihydrolipoyl transaeylase) of the branched-chain a-ketoacid dehydrogenase (BCKAD) complex was determined. In the region encoding the mature E2 subunit protein, both the nucleotide composition and predicted ammo acid sequence are highly conserved between murine, human, and bovine species. In contrast, the 5' sequence encoding the amino-terminal preprotein sequence and 3' untranslated region are less well conserved. The 3'-noncoding region contains sequences highly homologous to the rodent Bl repeat elements, which are related to human Alu repeat sequences. This finding is similar to the presence of three Alu repeat sequences in the 3'-noncoding region of human E2 cDNA.