Nitric oxide involved in signal transduction of Jasmonic acid-induced stomatal closure of Viciafaba L.

摘要

Nitric oxide (NO) and Jasmonic acid (JA) are two key signaling molecules involved in many and diverse biological pathways in plants. Growing evidence suggested that NO signaling interacts with JA signaling. In this work, Our experiment showed that NO exists in guard cell of Vicia faba L., and NO is involved in signal transduction of JA-induced stomata closuring: ( i ) JA enhances NO synthesis in guard cell; (ii) both JA and NO induced stomata! closure, and had dose response to their effects; (iii) there are syner-getic correlation between JA and lower NO concentration in regulation of stomatal movement; (iv) JA-lnduced stomatal closure was largely prevented by 2-phenyl-4,4,5,5-tetrame-thylimidazoline-1-oxyl-3-oxide (PTIO), a specific NO scavenger. An inhibitor of NO synthase (NOS) in mammalian cells, N~G-nitro-L-Arg-methyl eater (L-NAME) also inhibits plant NOS, repressing JA-induced NO generation and JA-induced stomatal closure. We presumed that NO mainly comes from NOS after JA treatment.