Genetic analysis and mapping of rice (Oryza saliva L.) male sterile (OsMS-L) mutant

摘要

A rice male-sterile mutant OsMS-L of japonica eultivar 9522 background, was obtained in M4 population treated with ~(60)Co y-Ray. Genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive gene. Results of tissue section showed that at microspore stage, OsMS-L tapetnm was retarded. Then tapetal cells expanded and microspores degenerated. No matured pollens were observed in OsMS-L anther locus. To OsMS-L locus, an F_2 population was constructed from the cross between the OsMS-L (japonica) and LongTeFu B(indicd). Firstly, the OsMS-L locus was roughly mapped between two SSR markers, RM1D9 and RM7562 on chromosome 2. And then eleven polymorphic markers were developed for farther fine fine-mapping. At last the OsMS-L locus was mapped between the two InDel markers, Lhs10 and Lhs6 with genetic distance of 0.4 cM, respectively. The region was delimited to 133 kb. All these results were useful for further cloning and functional analysis of OsMS-L.