On the role of carbohydrate-protein highly selective interactions in the biological activity of glycoconjugates: Grifola frondosa (Fr.) S.F. Gray lectin binding to specific and non-specific antibodies

摘要

Both quantitative and qualitative estimations of the basidiomycete Grifola frondosa lectin binding to the specific and non-specific polyclonal rabbit antibodies were attempted. The lectin complexation with homological antibodies was shown to be characterized by greater binding constants as compared to non-homological antibodies. Therewith the values of changes in standard free energy a dagger G (0) displaying a strength of both complexes were essentially the same. The data obtained testify to universality of biospecific reactions "antigen-antibody" and "lectin-carbohydrate" at a molecular level, and could give new insight into the phenomenon of these biospecific interactions. The actual specificity in the above bio-recognition processes could differ from the results of in vitro assays using lectins, and, therefore, should be interpreted carefully when concluding on the lectins behaviour in living systems.