Use of a chemical blocking agent against non-specific binding of an antibody specific for terminal deoxynucleotidyl transferase in biological specimens during immunoassays

摘要

A chemical agent is provided or significantly preventing or blocking non-specific staining or binding of an antibody specific for Terminal Deoxynucleotidyl Transferase (TdT) during immunofluorescent or im­munoperoxidase assay procedures. These procedures in­clude both immunofluorescent and immunohistochemical staining of samples followed by flow cytometric and/or microscopic analysis, respectively. The invention is practiced by selective use of casein introduced into the assay procedures at an appropriate interval prior to analysis using a labelled or tagged monoclonal antibody specific to a TdT epitope. The casein util­ized successfully was obtained from a large variety of sources and includes the use of a non-fat milk product.