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CHEMICAL BLOCKING AGENT AGAINST NON-SPECIFIC BINDING OR STAINING OF AN ANTIBODY SPECIFIC FOR TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE IN BIOLOGICAL SPECIMENS DURING IMMUNOASSAY
CHEMICAL BLOCKING AGENT AGAINST NON-SPECIFIC BINDING OR STAINING OF AN ANTIBODY SPECIFIC FOR TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE IN BIOLOGICAL SPECIMENS DURING IMMUNOASSAY
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机译:免疫测定过程中针对生物样本中末端脱氧核糖转移酶特异性抗体的非特异性结合或染色的化学封闭剂
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摘要
A chemical agent is provided for significantly preventing or blocking non-specific staining or binding of an antibody specific for Terminal deoxynucleotidyl Transferase (TdT) during immunofluorescent or immunoperoxidase assay procedures. These procedures include both immunofluorescent and immunohistochemical staining of samples followed by flow cytometric and/or microscopic analysis, respectively. The invention is practiced by selective use of casein introduced into the assay procedures at an appropriate interval prior to analysis using a labelled or tagged monoclonal antibody specific to a TdT epitope. The casein utilized successfully was obtained from a large variety of sources and includes the use of a non-fat milk product.
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