CHEMICAL BLOCKING AGENT AGAINST NON-SPECIFIC BINDING OR STAINING OF AN ANTIBODY SPECIFIC FOR TERMINAL DEOXYNUCLEOTYDAL TRANSFERASE IN BIOLOGICAL SPECIMENS DURING IMMUNOASSAY

摘要

ABSTRACTA chemical agent is provided for significantly preventingor blocking non-specific staining or binding of an antibodyspecific for Terminal Deoxynucleotydal Transferase (TdT) duringimmunofluorescent or immunoperoxidase assay procedures. Theseprocedures include both immunofluorescent and immunohistochemi-cal staining of samples followed by flow cytometric and/or mi-croscopic analysis, respectively. The invention is practiced byselective use of casein introduced into the assay procedures atan appropriate interval prior to analysis using a labeled ortagged monoclonal antibody specific to a TdT epitope. Thecasein utilized successfully was obtained from a large varietyof sources and includes the use of a non-fat milk product.-1-