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Diversity and biotechnological potential of culturable bacteria from Brazilian mangrove sediment

机译:巴西红树林沉积物中可培养细菌的多样性和生物技术潜力

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摘要

Mangrove ecosystems are environments subject to substantial degradation by anthropogenic activities. Its location, in coastal area, interfacing the continents and the oceans makes it substantially important in the prospection for biotechnological applications. In this study, we assessed the diversity of culturable bacteria present over the seasons at two depths (0-10 and 30-40 cm) in a mangrove sediment and in a transect area from the land to the sea. In total, 238 bacteria were isolated, characterized by Amplified Ribosomal DNA Restriction Analysis (ARDRA) and further identified, by Fatty Acid Methyl Esther (FAME-MIDI), into the orders of Vibrionales, Actinomycetales and Bacillales. Also the ability of the isolates in producing economically important enzymes (amylases, proteases, esterases and lipases) was evaluated and the order Vibrionales was the main enzymatic source.
机译:红树林生态系统是由于人为活动而严重退化的环境。它的位置位于沿海地区,与各大洲和海洋相接,因此在生物技术应用前景中非常重要。在这项研究中,我们评估了红树林沉积物和从陆地到海洋的横断面两个深度(0-10和30-40 cm)两个季节中存在的可培养细菌的多样性。总共分离出238个细菌,以扩增的核糖体DNA限制性分析(ARDRA)为特征,并通过脂肪酸甲基Esther(FAME-MIDI)进一步鉴定为Vibrionales,Actinomycetales和Bacillales。还评估了分离物产生经济上重要的酶(淀粉酶,蛋白酶,酯酶和脂肪酶)的能力,并且以弧菌为主要酶源。

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