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Genomic evidence that simian virus 2 and six other simian picornaviruses represent a new genus in Picornaviridae

机译:猿猴病毒2和其他六种猿猴小核糖核酸病毒病毒代表Picornaviridae的新属的基因组证据

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Analysis of the VP1 capsid protein coding region of simian virus (SV)2, SV16, SV18, SV42, SV44, SV45, and SV49 demonstrates that they are clearly distinct from members of the Enterovirus genus and from members of other existing picornavirus genera. To further, characterize this group of viruses and to clarify their classification within the Picornaviridae, we have determined the complete genomic sequence of SV2 (8126 nucleotides). The genome was typical of members of Picornaviridae, encoding a single open reading frame. The putative polyprotein contained typical picornavirus protease cleavage sites, yielding mature proteins homologous to each of the known picornavirus proteins. SV2 contained an amino-terminal extension of the reading frame, which was analogous to the leader protein of members of the Aphthovirus, Cardiovirus, Erbovirus, Kobuvirus, and Teschovirus genera, but there was no significant amino acid homology with any of these known leader proteins. The 2A protein also aligned poorly with the 2A proteins of other picornaviruses. The deduced amino acid sequences of the SV2 structural and nonstructural proteins were related to but phylogenetically distinct from those of enteroviruses and human rhinoviruses. The major distinguishing features of SV2 were the presence of a type 2 internal ribosome entry site in the 5'-NTR, a putative leader protein encoded upstream of the structural proteins, and an unusually large 2A protein. On the basis of the molecular analysis, we propose that SV2, SV16, SV18, SV42, SV44, SV45, SV49, and porcine enterovirus 8 be classified as members of a new genus in Picornaviridae and that SV2 (strain 2383) be designated as the type strain.
机译:猿猴病毒(SV)2,SV16,SV18,SV42,SV44,SV45和SV49的VP1衣壳蛋白编码区的分析表明,它们明显不同于肠病毒属成员和其他现有小核糖核酸病毒属成员。为了进一步表征这组病毒并阐明其在Picornaviridae中的分类,我们确定了SV2的完整基因组序列(8126个核苷酸)。该基因组是Picornaviridae成员的典型基因组,编码单个开放阅读框。推定的多蛋白含有典型的小核糖核酸病毒蛋白酶切割位点,产生与每种已知的小核糖核酸病毒蛋白同源的成熟蛋白。 SV2包含阅读框的氨基末端延伸,类似于鱼鳞病毒,心病毒,erbovirus,Kobuvirus和Teschovirus属成员的前导蛋白,但与这些已知的前导蛋白均无显着的氨基酸同源性。 2A蛋白也与其他小核糖核酸病毒的2A蛋白排列不佳。推导的SV2结构蛋白和非结构蛋白的氨基酸序列与肠道病毒和人鼻病毒的氨基酸序列相关,但在系统发育上不同。 SV2的主要区别特征是5'-NTR中存在2型内部核糖体进入位点,结构蛋白上游编码的推定前导蛋白和异常大的2A蛋白。在分子分析的基础上,我们建议将SV2,SV16,SV18,SV42,SV44,SV45,SV49和猪肠病毒8归类为Picornaviridae的新属成员,并将SV2(菌株2383)指定为类型应变。

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