IDENTIFICATION OF AN ALPHA-D-MANP-(1-]8)-KDO DISACCHARIDE IN THE INNER CORE REGION AND THE STRUCTURE OF THE COMPLETE CORE REGION OF THE LEGIONELLA PNEUMOPHILA SEROGROUP 1 LIPOPOLYSACCHARIDE

摘要

A disaccharide alpha-D-mannopyranosyl-(1 --> 8)-3-deoxy-D-manno-octulosonic acid [alpha-D-Manp-(1 --> 8)-Kdo] was released by mild acid degradation of Legionella pneumophila serogroup 1 (strain Philadelphia 1) lipopolysaccharide (LPS) and identified using NMR spectroscopy and GLC-MS of derived products. These data, together with methylation analysis of the native LPS and previously reported data [Y.A. Knirel, H. Moll, and U, Zahringer, Carbohydr. Res., 293 (1996) 223-234], allowed elucidation of the complete core region of the LPS as having the following nonasaccharide structure: [GRAPHICS] (C) 1997 Elsevier Science Ltd. [References: 22]