Optimization of extraction technology of Se-enriched Hericium erinaceum polysaccharides by Box-Behnken statistical design and its inhibition against metal elements loss in skull

摘要

This article described the application of response surface methodology (RSM) to the development of a procedure for extraction of Se-enriched Hericium erinaceum polysaccharides. A Box-Behnken matrix was used to find optimal conditions for the procedure through a response surface study. Four variables "extraction time; extraction temperature; ratio of liquid to solid and extraction number" were regarded as factors in the optimization study. The optimal extraction conditions for the Se-enriched H. erinaceum polysaccharides were: extracting time 115min, extracting temperature 98 °C, ratio of liquid to solid 4, and extracting number 4. Compared with untreated cells, administration of Se-enriched H. erinaceum polysaccharides improved BMC and BMD in experimental rats. These results showed that Se-enriched H. erinaceum polysaccharides could inhibit metal elements loss in rats' skull.