Alone and complemented wastewater as a substitute in an 'in vitro' culture media of coffee embryos

摘要

This work was carried out in Provincial Enterprise from Hydraulical Resources of Santiago de Cuba and a Genetic Laboratory from Central Research Station of Coffee and Cacao, Tercer Frente, Santiago de Cuba province, from February to June of 1996 period. The "in vitro" culture of Coffea arabica L. 'Catuai Rojo' var. embryos was made as substitute the traditionally culture media using wastewater of Neat Enterprise of Santiago de Cuba as alone and mixed form with coconut milk and ammonium nitrate. Mainchemical components were analysed at 19, 27, 36 and 44 days after culturing, the stem and root length were evaluated. The statistical analysis showed a significative difference between treatments. The best behaviour culture media were wastewater complemented with 500 mg.l~(-1) or 1000 mg.l~(-1) of ammonium nitrate.