Method for assessing the effectiveness of embryo culture media employed in processes of fertilization and in vitro culture, based on the ability of the embryos to bind epidermal growth factor

摘要

The present invention contemplates a method for evaluating the effectiveness of embryo culture media employed in processes of fertilization and in vitro culture, based on the ability of the embryos to bind epidermal growth factor, comprising the following steps: a) obtaining non-human embryos by in vitro or in vivo fertilization, b) Development of embryos to the blastocyst stage, c) culture of embryos obtained in a) and b) in a culture medium with epidermal growth factor conjugated to fluorochrome d) Measurement of the amount of growth factor conjugated to fluorochrome, attached to the surface of embryos cultured in c), where a higher intensity of fluorescence is related to optimal growing conditions.