Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS II. LC-MS/MS analysis (Review)

摘要

The first section of Part II describes the current strategies for mass spectrometric (MS) detection in the selected reaction monitoring (SRM) mode which has become the method of choice to quantify peptides. We then discuss the selection of signature peptides, SRM transitions and labeled internal-standard peptides to obtain the best assay selectivity. We also present improved assay selectivity on triple-quadrupole linear ion trap using MS~3 and differential mobility MS. We dedicate the final section to alternative approaches based on high-resolution data-independent acquisition.