Tyrosine hydroxylase protein expression in ventral nerve cord of Neotropical freshwater crab

摘要

Given the importance of catecholamines in coordinating physiological and behavioral responses in brachyurans, the present study was designed to investigate the distribution of tyrosine hydroxylase (TH)-positive cells and fibers in the ventral nerve cord of Dilocarcinus pagei the Neotropical freshwater crab. TH immunoreactivity was visualized in adult crabs of both sexes, during the intermolt period. We found TH-positive cells that have not been previously described in brachyurans. Specifically, we found a pair of TH-positive cells in the ventral region of the thoracic ganglion, and in ventral and dorsal regions of the abdominal (pleonic) ganglion, suggesting catecholaminergic modulation of claws' function and abdominal structures. In addition, great population of TH-positive cells was observed in the subesophageal ganglion, indicating conservation during evolution of catecholamines in this ganglion of decapods. Dopamine is present in cells and fiber tracts of brachyuran ventral nerve cord, projecting to endocrine, cardiac and digestive structures, suggesting widespread modulation and control of physiological functions and behavior. Dopamine plays a central role in movement and psychiatric disorders in humans. Information on dopaminergic function in the nervous system of invertebrates should improve the understanding of its function in more complex systems, such as human beings. (C) 2014 Elsevier Ltd. All rights reserved.