Effect of sperm number and oxygenation state of the storage media on invitro fertility of bovine sperm stored at ambient temperature

摘要

The effects of storage time and the oxygenation state of the storage medium on motility, viability, and in vitro fertility of stored diluted sperm were investigated. Oocytes collected from abattoir material were matured and fertilized in vitro on defined days with sperm stored for up to 11 days in a citrate-based commercial diluent. The proportions of oocytes fertilized and developing to the blastocyst stage were used to assess the quality of the stored semen. In vitro fertility of sperm. declined with storage time. There was no significant effect of the oxygenation state of the medium on in vitro fertility of stored sperm. Increased sperm-to-oocyte ratios resulted in a significant elevation of the proportion of oocytes fertilized on day 0 of storage and the proportion of fertilized oocytes developing to the blastocyst stage on days 0 and 3 of storage, suggesting some form of sperm competition or egg selection of sperm based on the ability of sperm to induce normal development.