In 1997, a radical idea challenged the individuality of plastids and their discrete nature in vascular plants. The idea was based on the flow of green fluorescent protein (GFP) between interconnected plastids through narrow tubules (K?hler et al., 1997), later called stromules (K?hler and Hanson, 2000). The initial observations suggested an interconnected-plastid network, but by 2000 it was made clear that such a network did not exist. However, the underlying concept of protein flow between interconnected plastids remained unchallenged until our publication in 2012 (Schattat et al., 2012a). In a commentary on the subject, Hanson and Sattarzadeh (2013) note that they had previously negated the idea of a plastid network and suggest that our investigations using a photoconvertible protein merely confirmed their earlier findings. We agree with several points made in the commentary, namely, that stromules emerging from a single plastid rarely form a connection to other independent plastids, and as such, protein movement between interconnected plastids likely does not have major biological significance to the plant cell.
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机译：1997年，一个激进的想法挑战了质体的个性及其在维管植物中的离散性。这个想法是基于绿色荧光蛋白（GFP）在相互连接的质体之间通过狭窄的小管（K？hler等，1997）流动，后来又称为基质（K？hler and Hanson，2000）。最初的观察结果表明存在一个互连的质体网络，但是到2000年，人们已经清楚地意识到这种网络不存在。但是，直到我们在2012年发表论文（Schattat等，2012a），相互连接的质体之间的蛋白质流动的基本概念仍然没有受到挑战。在关于该主题的评论中，Hanson和Sattarzadeh（2013）指出，他们之前已经否定了质体网络的想法，并暗示我们使用光可转换蛋白的研究仅证实了他们先前的发现。我们同意评论中的几点观点，即从单个质体出现的基质很少与其他独立质体形成连接，因此，相互连接的质体之间的蛋白质运动可能对植物细胞没有重要的生物学意义。
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