首页> 外文期刊>The journals of gerontology.Series A. Biological sciences and medical sciences >Changes in energy-regulated molecules in the trophocytes and fat cells of young and old worker honeybees (Apis mellifera)
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Changes in energy-regulated molecules in the trophocytes and fat cells of young and old worker honeybees (Apis mellifera)

机译:老年和老年工蜂(Apis mellifera)的滋养细胞和脂肪细胞中能量调节分子的变化

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Trophocytes and fat cells of honeybees (Apis mellifera) have been used for cellular senescence studies, but the changes in the expression, concentration, and activity of cellular energy-regulated molecules that occur with aging in worker bees is unknown. In this study, energy-regulated molecules were evaluated in the trophocytes and fat cells of young and old workers. The results showed that (i) adenosine monophosphate-activated protein kinase-α2 (AMPK-α2) expression increased with aging, whereas phosphorylated AMPK-α2 expression, the phosphorylated AMPK/AMPK ratio, and AMPK activity decreased with aging; (ii) adenosine diphosphate and adenosine triphosphate concentrations decreased with aging, the AMP concentration was unchanged, the adenosine diphosphate/adenosine triphosphate ratio did not change with aging, and the AMP/adenosine triphosphate ratio increased with aging; (iii) the cyclic AMP concentration decreased with aging, and cyclic AMP-specific phosphodiesterases activity increased with aging; (iv) silent information regulator 2 (Sir2) expression increased with aging, whereas its activity decreased with aging; and (v) peroxisome proliferator-activated receptor-α expression decreased with aging. These results show that the trophocytes and fat cells of young workers have higher cellular energy status and express higher levels of energy-regulated molecules than those of old workers and that aging results in a decline in the energy status of trophocytes and fat cells in worker honeybees.
机译:蜜蜂(Apis mellifera)的滋养细胞和脂肪细胞已经用于细胞衰老研究,但是随着工蜂衰老而发生的细胞能量调节分子的表达,浓度和活性变化尚不清楚。在这项研究中,评估了能量调节分子在年轻工人和老年人的营养细胞和脂肪细胞中的作用。结果表明:(i)腺苷一磷酸激活蛋白激酶-α2(AMPK-α2)的表达随衰老而增加,而磷酸化的AMPK-α2的表达,磷酸化的AMPK / AMPK比和AMPK的活性随衰老而降低; (ii)二磷酸腺苷和三磷酸腺苷的浓度随老化而降低,AMP浓度不变,二磷酸腺苷/三磷酸腺苷的比例随老化而变化,而AMP /三磷酸腺苷的比率随老化而增加; (iii)环状AMP浓度随老化而降低,环状AMP特异性磷酸二酯酶活性随老化而增加; (iv)沉默信息调节因子2(Sir2)的表达随衰老而增加,而其活性随衰老而降低; (v)过氧化物酶体增殖物激活的受体-α表达随衰老而降低。这些结果表明,年轻工人的营养细胞和脂肪细胞具有比老年人更高的细胞能量状态,并表达更高水平的能量调节分子,并且衰老导致工人蜜蜂的营养细胞和脂肪细胞的能量状态下降。 。

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