Expression Profiling of Mouse Placental Lactogen II and Its Correlative Genes Using a cDNA Microarray Analysis in the Developmental Mouse Placenta

摘要

The placenta is a highly differentiated organ essential for embryonic growth anddevelopment. In order to search for key molecules that are associated with mouse placental lactogenII (mPL-II) gene expression, we applied mouse cDNA microarray analysis to RNAs extracted fromplacentae on days 10, 12, 14, 16 and 18 of pregnancy. Changes in gene expression were categorizedbetween days 10 and 12,12 and 14,14 and 16 and 16 and 18 of pregnancy. After microarray analysis,which had a minimum detectable fold change for differential expression of 2, we selected 10 genes,Apoa2, Apoc2, Ceacaml4, Cregl, Fmol, Igf2, Slc2al, Spink3, Spil-1 and Tpbpa, exhibiting aexpression pattern similar to the mPL-II gene. Furthermore, we performed real-time PCR analysisand in situ hybridization (ISH) to find correlative expression genes for the mPL-II gene. From theseresults, we identified a resemblance in gene expression between mPL-II and Igf2 and selected thesegenes for performance of double-fluorescence immunohistochemical staining. We colocalized theseproteins in labyrinthine trophoblast cells. These results strongly suggest that the expression of mPL-IIand Igf2 is highly related to placental development in mice. This large-scale identification of genesregulated during placentogenesis assists in further elucidation of the molecular basis ofextraembryonic development and function.