Effects of Medium, Temperature and pH on Mycelial Growth and Cellulase Activity of Ectomycorrhizal Fungi from Korean Forests

摘要

Mycelial growth of ectomycorrhizal fungi (27 strains of 8 species) collected from Korean forests was observed on various culture conditions (media, temperature, pHs). After 60 days of incubation, all strains grown on potato dextrose agar (PDA) and modified Melin-Norkran's agar (MMNA), whereas no mycelial growth was observed on malt extract agar (MEA) or sabouraud dextrose agar (SDA) in some strains including Tricholoma ma-tsutake. Mycelial growth on PDA was poor at high temperature (30°C) than the low temperature (10°C). The optimal temperature on PDA and pH in potato dextrose broth (PDB) for mycelial growth in most strains were 20-25°C and pH 4-5, respectively. All strains tested showed the carboxymethyl cellulase (CM-cellulase) activity and themaximal cellullase activity was expressed by the mycelium of T. matsutake (KFRI 1266) on the CMC agar plate with pH 5.0.