Protective Effect of Polyethylene Glycol against Prolonged Cold Ischemia and Reperfusion Injury: Study in the Isolated Perfused Rat Kidney

摘要

The purpose of this study was to evaluate an intracellular solution with polyethylene glycol (PEG, molecular weight 20,000) as an impermeant, compared with University of wis- consin (UW) and Euro-Collins (EC) solutions, after a 48-h cold storage (CS). The normothermic isolated perfused rat kidney (IPK) technique was used to assess renal function after CS. Five groups were studied: a control group (immediately reperfused, n = 10); one that received EC (n = 16); one that received UW (n = 16); and two that each received an intracellular (IC) solu- tion, one with PEG (ICPEG, n = 16) and one without PEG (IC, n = 16). The perfusion flow rate was significantly greater in the PEG group and correlated with less significant cellular and interstitial edema and lower renal vascular resistance than in the IC, EC, and UW groups. Glomerular filtration rate was significantly higher in the PEG group during reperfusior the IC, EC, and UW groups. Proximal tubular functiol more efficient with PEG: fractional sodium reabsorpt total sodium reabsorption were significantly greater reperfusion in the PEG group than in the IC, EC, a groups. Of greater interest is the protective effect of I lipid peroxidation, which reflects ischemia/reperfusion d The second major effect is the dramatic A TP restoratior reperfusion, which outlines the preservation of oxidativ phorylation after preservation by ICPEG. These results c ported by histological studies, particularly concernin! border and mitochondrial preservation. Our results indic PEG is promising for cold ischemia and reperfusion injl tection.