Simultaneous LC-MS-MS analysis of danshensu, salvianolic acid B, and hydroxysafflor yellow A in beagle dog plasma, and application of the method to a pharmacokinetic study of Danhong lyophilized powder for injection

摘要

A reliable and sensitive liquid chromatographic-tandem mass spectrometric method, with rutin as internal standard, has been developed and validated for simultaneous determination of danshensu, salvianolic acid B (SAB), and hydroxysafflor yellow A (HSYA) in beagle dog plasma. Plasma samples spiked with the analytes were extracted by solid-phase extraction and the analytes were separated on a 250 x 4.6 mm i.d., 5-mu m particle, C-18 column with methanol-acetonitrile-0.5% formic acid 20:25:55 (v/v) as mobile phase at a flow rate of 1 mL min(-1). LC-MS-MS analysis was performed with a Finnigan TSQ triple-quadrupole tandem mass spectrometer operated in negative-ion selected-reaction-monitoring mode, using electrospray ionization. The accuracy and precision of the method were acceptable and linearity was good over the range 20-4,000 ng mL(-1) for danshensu, 50-10,000 ng mL(-1) for SAB, and 10-2,000 ng mL(-1) for HSYA. The method was successfully applied to a pharmacokinetic study of a traditional Chinese medicinal preparation, Danhong lyophilized powder for injection.