TLC Determination of Amitriptyline HCI, Trifluoperazine HCI, Risperidone and Alprazolam in Pharmaceutical Products

摘要

A simple, sensitive, and precise thin layer chromatographic (TLC) method for simultaneous analysis of psychopharmacological drugs like amitriptyline HCI, trifluoperazine HCI, ris-peridone and alprazolam in their single dosage forms has been developed, validated, and used for determination of the compounds in commercial pharmaceutical products. The TLC separation was carried out on Merck TLC aluminium sheets of silica gel 60 F254 using carbon tetrachloride:acetone:triethylamine (8:2:0.3, v/v/v), as mobile phase. Densitometric measurements of their spots were achieved at 250 nm over the concentration range for amitriptyline HCI (50-1,200 ng spot~(-1)), trifluoperazine HCI (50-1,200 ng spot~(-1)), risperidone (100-2,400 ng spot~(-1)) and alprazolam (25-600 ng spot~(-1)). Limit of detection (LOD) for amitriptyline HCI (20 ng spot~(-1)), trifluoperazine HCI (20 ng spot~(-1)), risperidone (40 ng spot~(-1)) and alprazolam (5 ng spot~(-1)) was obtained. The study showed that TLC was sensitive and selective for determination of amitriptyline HCI, trifluoperazine HCI, risperidone and alprazolam using a single mobile phase. This proposed method is able for simultaneous determination of psychopharmacological drugs and also applicable for analysis of pharmaceutical formulations.