NGS Sequence Data with Too Few Reads for Adequate Analysis May Reflect the Presence of an Amplified Gene Consuming Reagents Rather than Poor Quality of DNA

Plambeck J.; Cushman-Vokoun A.; Zhang W.; Bridge J. A.; Greiner T. C.

首页> 外文期刊>The Journal of molecular diagnostics: JMD >NGS Sequence Data with Too Few Reads for Adequate Analysis May Reflect the Presence of an Amplified Gene Consuming Reagents Rather than Poor Quality of DNA

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NGS Sequence Data with Too Few Reads for Adequate Analysis May Reflect the Presence of an Amplified Gene Consuming Reagents Rather than Poor Quality of DNA

机译：NGS序列数据的读数不足以进行足够的分析，可能反映了存在扩增的基因消耗试剂，而不是DNA的质量较差

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《The Journal of molecular diagnostics: JMD 》 |2016年第6期| 共2页
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Plambeck J.; Cushman-Vokoun A.; Zhang W.; Bridge J. A.; Greiner T. C.;
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中图分类临床医学 ;
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1. NGS Sequence Data with Too Few Reads for Adequate Analysis May Reflect the Presence of an Amplified Gene Consuming Reagents Rather than Poor Quality of DNA [J] . Plambeck J., Cushman-Vokoun A., Zhang W., The Journal of molecular diagnostics: JMD . 2016 ,第6期

机译：NGS序列数据的读数不足以进行足够的分析，可能反映了存在扩增的基因消耗试剂，而不是DNA的质量较差
2. Genetic diversity analysis ofCorynespora cassiicolaisolates on the rubber tree (Hevea brasiliensis) in Vietnam using ribosomal DNA internal transcribed spacer (rDNA-ITS) sequences and sequence-related amplified polymorphism (SRAP) [J] . Hieu N. D., Nghia N. A., Uyen N. T. K., Journal of Rubber Research . 2020 ,第3期

机译：使用核糖体DNA内转录的间隔（RDNA-ITS）序列和序列相关扩增多态性（SRAP）越南橡胶树（HEVEA BRASILIENSIS）对越南橡胶树（HEVEA BRASILIENSIS）的遗传多样性分析
3. OPTIMIZATION OF CYCLE SEQUENCING DNA BASE WITH REAGENTS BIGDYE PGEM USING DNA SEQUENCER GENETIC ANALYSIS AUOTOMATIS ABI PIRSM 310 [J] . HARUMI YUNIARTI, BAMBANG CHOLIS S, ASTRI RINANTI NUGROHO Asian Journal of Microbiology, Biotechnology and Environmental Science . 2016 ,第1期

机译：DNA测序仪遗传分析AUTOTOMATIS ABI PIRSM 310用试剂BIGDYE PGEM优化循环测序DNA碱基
4. Reads in NGS Are Distributed over a Sequence Very Inhomogeneously [C] . Michael Sadovsky, Victory Kobets, Georgy Khodos, International work-conference on bioinformatics and biomedical engineering . 2019

机译：NGS中的读段非常不均匀地分布在序列上
5. Random amplified polymorphic DNA and sequence characterized amplified regions for studies of genetic diversity and downy mildew resistance in cucumber. [D] . Horejsi, Thomas. 1998

机译：随机扩增的多态性DNA和序列表征的扩增区域，用于研究黄瓜的遗传多样性和霜霉病抗性。
6. Presence of Bacterial Phage-Like DNA Sequences in Commercial Taq DNA Polymerase Reagents [O] . Tamara Newsome, Bing-Jie Li, Nianxiang Zou, 2004

机译：Taq DNA聚合酶试剂中存在细菌噬菌体样DNA序列
7. Phylogenetic analysis of Cryptosporidium isolates from captive reptiles using 18S rDNA sequence data and random amplified polymorphic DNA analys [O] . Morgan U.M., Xiao L., Fayer R., 1999

机译：利用18S rDNA序列数据和随机扩增多态性DNA分析技术对圈养爬行类隐孢子虫分离株进行系统发育分析
8. Cryptic Species in the Anopheles (Nyssorhynchus) albitarsis (Diptera: Culicidae) Complex: Incongruence Between Random Amplified Polymorphic DNA- Polymerase Chain Reaction Identification and Analysis of Mitochondrial DNA COI Gene Sequences. [R] . Lehr, M. A., Kilpatrick, C. W., Wilkerson, R. C., 2005

机译：按蚊（Nyssorhynchus）albitarsis（双翅目：蚊科）复合体中的隐蔽物种：随机扩增多态DNa-聚合酶链反应鉴定和线粒体DNa COI基因序列分析之间的不一致。

NGS Sequence Data with Too Few Reads for Adequate Analysis May Reflect the Presence of an Amplified Gene Consuming Reagents Rather than Poor Quality of DNA

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