Effects of exogenous H2S on the germination of tomato seeds under nitrate stress

摘要

In this study, the effects of the exogenous H2S donor, NaHS, on the germination of tomato seeds under nitrate (NO3-) ion stress were investigated. The results showed that under increased nitrate ion stress (range 0 - 200 mM), the germination percentage of tomato seeds decreased from 92.3% to 1.0%. The application of NaHS, up to 100 mu M, alleviated the inhibitory effect of nitrate stress by enhancing seed germination up to 1.3-fold compared to the nitrate stress treatment alone. Further study showed that malondialdehyde concentrations and the accumulation of reactive oxygen species declined significantly after all NaHS treatments. Amylase, superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase activities increased significantly after 100 mu M NaHS treatment. In addition, nitric oxide (NO) fluorescence increased when NaHS was added to the nitrate solution. Our results suggest that exogenous H2S can alleviate the damage caused by nitrate stress, possibly through increasing anti-oxidant enzyme activities, and may have a interaction with NO.