A new method for the detection and elimination of Hydrangea ringspot virus.

Toth E. K.; Kriston E.; Mathe A.; Jambor-Benczur E.

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A new method for the detection and elimination of Hydrangea ringspot virus.

机译：一种检测和消除绣球花环斑病毒的新方法。

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The complete eradication of Hydrangea ringspot virus (HRSV) from Hydrangea by in vitro pre-treatment with ribavirin and meristem tip culture is reported. A sensitive RT-PCR method for the detection of HRSV in newly-formed regenerants was developed. Total nucleic acid extracts from HRSV-infected Hydrangea leaves diluted 105-fold gave a positive reaction using this method.

机译：据报道，通过利巴韦林和分生组织尖端培养物的体外预处理可以完全根除绣球花中的绣球花环斑病毒（HRSV）。建立了一种灵敏的RT-PCR方法，用于检测新形成的再生物中的HRSV。使用此方法，将HRSV感染的八仙花（Ii）绣球叶子稀释10倍（sup）5 倍，总核酸提取物产生阳性反应。

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《The Journal of Horticultural Science & Biotechnology》 |2012年第1期|共4页
作者
Toth E. K.; Kriston E.; Mathe A.; Jambor-Benczur E.;
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正文语种 eng
中图分类园艺;
关键词
antiviral agents; in vitro culture; leaves; nucleic acids; plant disease control; plant pathogens; plant viruses; reverse transcriptase PCR; ribavirin; tissue culture; Flexiviridae; Hydrangea; Hydrangea ringspot virus; Potexvirus; positive-sense ssRNA viruses; ssRNA viruses; RNA viruses; viruses; Hydrangeaceae; Cornales; Rosales; dicotyledons; angiosperms; Spermatophyta; plants; eukaryotes; Potexvirus; Flexiviridae; phytopathogens; reverse transcriptase polymerase chain reaction; RT-PCR; tribavirin; viruses of plants;

机译：抗病毒药物;体外培养;叶;核酸;植物病害防治;植物病原体;植物病毒;逆转录酶PCR;利巴韦林;组织培养;弯曲病毒科;绣球;绣球花环斑病毒;痘病毒;正感ssRNA病毒;ssRNA病毒;RNA病毒;病毒;绣球科;科纳莱斯;罗莎属;双子叶植物;拟子植物;精子植物;植物;真核生物;痘病毒;弯曲病毒科;植物病原体;逆转录酶聚合酶链反应;RT-PCR;特立巴韦林;植物病毒;

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1. A new method for the detection and elimination of Hydrangea ringspot virus. [J] . Toth E. K., Kriston E., Mathe A., The Journal of Horticultural Science & Biotechnology . 2012,第1期

机译：一种检测和消除绣球花环斑病毒的新方法。
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3. Inter-laboratory evaluation of a duplex RT-PCR method using crude extracts for the simultaneous detection of Prune dwarf virus and Prunus necrotic ringspot virus [J] . Massart S., Brostaux Y., Barbarossa L., European Journal of Plant Pathology . 2008,第4期

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A new method for the detection and elimination of Hydrangea ringspot virus.

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