ISOLATION OF A GENE PREFERENTIALLY EXPRESSED IN MATURE ANTHERS OF RICE (ORYZA SATIVA L.)

摘要

Using monoclonal antibodies raised against pollen-specific proteins, we have isolated a cDNA clone, designated Ory-Cl from a rice anther cDNA expression library. A transcript corresponding to the Ory-Cl gene showed preferential expression in anthers. This transcript was not detected in any vegetative tissues analysed. RNA gel blot analysis of different developmental stages of anthers showed that the Ory-Cl gene is expressed at later stages of pollen development. In situ hybridisation showed that the Ory-Cl transcript is only present in mature pollen. [References: 20]