Effect of Holding of Porcine Semen Before Cooling on Motility and Viability of Spermatozoa

摘要

Experiment was conducted to find out the effect of holding of porcine semen for different periods at 24 deg C on motility and live sperm count at different stages of processing during freezing and post-thawing. It was observed that sperm motility andlive sperm count were significantly higher in 1 h and 3 h holding groups up to the stage of equilibration than in 5 and 20 h holding groups. However, after thawing significantly higher sperm motility and liver sperm count was obtained in 3 h holding group than in 1, 5 and 20 h holding. Therefore, it was concluded that 3 h holding of semen prior to freezing would give better protection to spermatozoa during freezing.