Change in Viability, Motility and Membrane Integrity of Spermatozoa After Equilibration and Thawing in BHT Added Bovine Semen

摘要

This study was conducted to evaluate effect of butylated hydroxytoluene (BHT) on reduction in semen quality (progressive motility, viability and membrane stability) from dilution to equilibration and thawing. Thirty-six ejaculates from two bulls were individually collected and extended with glycerolated egg yolk Tris diluter. Samples were divided into three groups according to BHT concentration as 0 mM (C) 0.5 mM (T1) and 1.0 mM (T2). Reduction in the progressive motility, sperm viability and membrane integrity was observed from Stage I (after dilution) to Stage II (post equilibration) and Stage III (post thaw). Decrease in percentage individual motility from Stage I to Stage II, Stage II to Stage III and Stage I to III was lowest in group T2 though it was non-significant. Decrease in per cent viability from Stage I to Stage II, Stage II to Stage III and Stage I to Stage III indicated lesser loss in viability in group T2. Percent decrease in membrane stable spermatozoa from Stage I to Stage II, Stage II to Stage III and Stage I to Stage III was minimum in group T2. Addition of 1mM of BHT in semen dilutor showed better results as fall in sperm viability, progressive motility and membrane integrity was least from post dilution to post equilibration and post thaw though it was non-significant. Thus experiments to observe the effect of BHT on post thaw semen quality should be conducted at large scale.