Amplifications and RFLP of exon 2 of MHC-DRB3 locus in livestock species.

摘要

The restriction fragment length polymorphism of exon 2 of MHC DRB3 locus in Rathi cattle, Magra sheep and French donkey was studied using Hinf I, Rsa I and Hae III. restriction enzymes. A fragment of 308 bp was successfully amplified in cattle (BoLA), donkey (ELA) and sheep (OLA). The digestion of BoLA-DRB3 fragment with Hinfi revealed heterozygous status at this locus in all the animals under investigation. The molecular size of fragments were 261, 47 and 167, 94 and 47 bp. Analysis of ELA (221 and 87 bp) and OLA DRB3 (212 and 96 bp) fragments revealed homozygous status at this locus. Digestion with HaeIII in donkey resolved two fragments of 169 and 139 bp. Rsa I revealed heterozygous status in all three species. No intra-species variation was detected. The fragments observed in cattle and sheep were of the same size (308 and 160, 81, 67 bp) indicating the genetic similarity in the two species. In French donkeys the fragments resolved were of 308 and 138, 85, 85 bp.