首页> 外文期刊>The British Journal of Nutrition >Probiotic attributes of Lactobacillus strains isolated from food and of human origin.
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Probiotic attributes of Lactobacillus strains isolated from food and of human origin.

机译:从食物和人类来源分离的乳酸杆菌菌株的益生菌特性。

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Lactobacilli isolated from various sources were identified on the basis of 16S-23S rRNA gene intergenic region amplification and subsequent sequencing of the smaller intergenic region. An in vitro analysis of probiotic properties including binding, ability to tolerate different concentrations of bile, survival in acidic buffer and antimicrobial activity of four different isolates and two standard strains (Lactobacillus plantarum American Type Culture Collection (ATCC) 8014 and L. rhamnosus GG (LGG)) was carried out. The ability of each isolate to stimulate Caco-2 cells, human peripheral blood mononuclear cells (PBMC) and THP-1 cells resulting in immunomodulation of these cells was analysed. Isolates L. rhamnosus CS25 and L. delbrueckii M and standard strain ATCC 8014 showed broad antimicrobial activity, and isolates CS25 (percentage of survival 6.9 % at pH 2.5, 5.1 % at pH 2.0) and L. plantarum CS23 (5.7 % at pH 2.5, 4.9 % at pH 2.0) have shown good tolerance to acidic pH. Isolate CS23 showed a good survival (14 %) after 2 h incubation in de Man, Rogosa and Sharpe (MRS) medium containing 3 % bile salts. Isolates CS23, CS25 and L. fermentum ASt1 could stimulate Caco-2 cells, human PBMC and THP-1 cells for a strong and varied immunomodulatory response in these cells. Though LGG showed poor antimicrobial activity as well as bile and acid tolerance, it was found to be the best binding strain tested. Child faecal isolate CS23 from the present study showed high binding ability (seventeen bacteria/Caco-2), high tolerance to acidic pH and bile salts and significant immunomodulation; therefore it is a good potential probiotic candidate
机译:根据16S-23S rRNA基因基因间区域扩增和较小基因间区域的后续测序,鉴定了从各种来源分离出的乳杆菌。益生菌特性的体外分析,包括四种不同菌株和两种标准菌株(植物乳杆菌美国典型培养物保藏中心(ATCC)8014和鼠李糖乳杆菌GG( LGG))。分析了每种分离物刺激Caco-2细胞,人外周血单个核细胞(PBMC)和THP-1细胞导致这些细胞免疫调节的能力。鼠李糖乳杆菌CS25和delbrueckii M菌株以及标准菌株ATCC 8014的分离物均显示出广泛的抗菌活性,并分离出CS25(pH 2.5时存活率为6.9%,pH 2.0时为5.1%)和植物乳杆菌CS23(pH 2.5时为5.7%)。 (pH 2.0时为4.9%)对酸性pH表现出良好的耐受性。在含有3%胆汁盐的de Man,Rogosa和Sharpe(MRS)培养基中孵育2 h后,分离株CS23表现出良好的存活率(14%)。分离CS23,CS25和发酵乳杆菌ASt1可以刺激Caco-2细胞,人PBMC和THP-1细胞在这些细胞中产生强大而多样的免疫调节反应。尽管LGG的抗微生物活性以及对胆汁和酸的耐受性很差,但发现它是测试的最佳结合菌株。来自本研究的儿童粪便分离物CS23具有高结合能力(十七种细菌/ Caco-2),对酸性pH和胆汁盐具有高度耐受性,并且具有明显的免疫调节作用。因此它是很好的潜在益生菌候选物

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