Status of oviduct of buffalo during follicular and luteal phases estrous cycle: A histological and histochemical perspective

摘要

Different segments of oviduct (mfundibulum, ampulla, isthmus andutero tubal junction) of six buffaloes each during follicular and luteal phases of estrous cycle were studied. The samples were collected in 10% NBF processed for paraffin sectioning werestained with hematoxylin and eosin, Masson's trichrome, Periodic Acid Schiff, alcian blue and PAS alcian blue. The mucosa was found thrown into longitudinal folds having primary, secondary and tertiary branches. Branching was more pronounced in infundibulum and ampulla as well as during the follicular phase as compared to luteal phase. The different segments were lined with columnar to pseudostratified columnar epithelium. The cells were ciliated and non ciliated type. In follicular phase, the epithelium showed strong PAS reaction which was concentrated in the supranuclear zone while during the luteal phase the reaction was moderate. The reaction was granular in nature. The epithelium was also strongly positive for alcian blue and PAS alcian blue during follicular phase. The propria submucosa, tunica muscularis and tunica serosa showed weak to mild reaction for PAS and alcian blue.