Modification of the mitochondrial F-1-ATPase epsilon subunit, enhancement of the ATPase activity of the IF1-F-1 complex and IF1-binding dependence of the conformation of the epsilon subunit

摘要

Treatment of bovine heart submitochondrial particles with a low concentration of 2-hydroxy-5-nitrobenzyl bromide (HNB), a selective reagent for the Trp residue of the epsilon subunit [Baracca, Barogi, Lenaz and Solaini (1993) Int. J. Biochem. 25, 1269-1275], enhances the ATP hydrolytic activity of the particles exclusively when the natural inhibitor protein IF1 is present. Similarly, isolated F-1 [the catalytic sector of the mitochondrial H+-ATPase complex (ATP synthase)] treated with the reagent has the ATPase activity enhanced exclusively if IF1 is bound to it. These experiments suggest that the modification of the epsilon subunit decreases the inhibitory activity of IF1, eliciting the search for a relationship between the epsilon subunit and the inhibitory protein. Certainly, a reverse relationship exists because HNB binds covalently to the isolated F-1 exclusively when the inhibitory protein is present. This finding is consistent with the existence of the a subunit in different conformational states depending on whether IF1 is bound to F-1 or not. Support for this assertion is obtained by measurements of the intrinsic phosphorescence decay rate of F-1, a probe of the Trp epsilon subunit conformation in situ [Solaini, Baracca, Parenti-Castelli and Strambini (1993) Eur. J. Biochem. 214, 729-734]. A significant difference in phosphorescence decay rate is detected when IF1 is added to preparations of F-1 previously devoid of the inhibitory protein. These studies indicate that IF1 and the a subunit of the mitochondrial F-1-ATPase complex are related, suggesting a possible role of the a subunit in the mechanism of regulation of the mitochondrial ATP synthase.