Cross-Linked Enzyme Aggregates in a Membrane Slurry Reactor Continuous production of 6-APA by enzymatic hydrolysis of penicillin

摘要

The use of Cross-Linked Enzyme Aggregates (CLEAs) in a Membrane Slurry Reactor (MSR), enabling highly efficient and cost-effective biotransformations, is reported. A membrane slurry reactor, which can easily be implemented by making minor modifications to an existing stirred tank reactor, retains the CLEA inside the reactor and combines the biotransformation and separation into a single operation. This MSR concept enables continuous production and the use of a broad range of catalyst particle sizes. High catalyst loadings, efficient catalyst use, long life time of the catalyst due to mild mechanical conditions and high volumetric productivities are some of the many advantages of this system. Proof of principle is demonstrated in a study of the industrially imnortnnt hvdmlvsis of important hydrolysis of penicillin G to 6-APA, catalysed by penicillin G amidase CLEA in an MSR.