GC-MS Determination of Fatty Acids in Arachidonic Acid High-Yield Strain Induced by Low-Energy Ion Implantation

摘要

With a novel mutation technique, low-energy ion beam implantation, which was originated in our laboratory, an arachidonic acid high-yield strain (Mortierella alpina) was obtained. The mycelium of this strain was dried and treated with boron fluoride-methanol solution. The fatty acids were determined by gas chromatography-mass spectrometry. The result showed that there were saturated and unsaturated fatty acids in the dried mycelium. The relative content of arachidonic acid was 70.2 % among all fatty acids. In addition, there were linoleic acid (3.985 %) and 7-linolenic acid (1.576 %) in the dried mycelium.