The influence of effective microorganisms on in vitro development of fungus Trichoderma harzianum occurring in button mushroom (Agaricus bisporus) crop.

摘要

The investigations were conducted in 2008. Effective microorganisms (EM), i.e. agents EM-A and EM-5 were added to PDA medium. Tested EM were applied alone and together (EM-A+EM-5). Control consisted medium with no EM added. Diameter of the T. harzianum colonies were measured after 2 and 4 days and sporulation of the fungus was determined 6 days after medium inoculation. The agents: EM-A and EM-5 applied together at a concentration of 100 mg/cm3 completely inhibited the development of T. harzianum. A strong inhibitory action reducing growth of the analyzed pathogen was recorded in the case of agents EM-A at a concentration of 100 mg/cm3 PDA and EM-A+EM-5 at a concentration of 50 mg/cm3. Inhibition of fungal growth was significant, and 4 days after medium inoculation it was 89.8% and 86.4%, respectively. At the same time sporulation of the fungus was significantly reduced.