OVINE ANTIVENOM FOR VIPERA AMMODYTES

摘要

This study describes in detail the procedure for producing in sheep antivenom serum for Viperaammodytes. This procedure includes also the reason and criteria for selection of animals intended tobe used for antivenom production. The most significant aspect of the immunization process isrepresented by splitting this into three distinct phases. First of this phases requires adsorbtion of thevenom on the alginate in order to gradually release into the animal body. It was tested also a simple method for purification and concentration of the immunoglobulin usingcaprylic acid. The antivenom efficacy of the crude serum and immunoglobulinic concentrate was investigated basedon the inhibitory action of the antivenom on the venom enzymes, particularly L-aminoacid-oxidase.Due to precision of instrumental techniques, the antienzymatic antivenom potency assay seems tobecome a very suitable strategy for better pharmaceutical and biochemical characterization ofantivenoms. Based on the less time consumption and resources reasonability, the method proposed in this study isvery appropriate for monitoring the immunoglobulin concentration during antivenom production.