首页> 外文期刊>Peptides: An International Journal >Postmenopausal increase in KiSS-1, GPR54, and luteinizing hormone releasing hormone (LHRH-1) mRNA in the basal hypothalamus of female rhesus monkeys.
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Postmenopausal increase in KiSS-1, GPR54, and luteinizing hormone releasing hormone (LHRH-1) mRNA in the basal hypothalamus of female rhesus monkeys.

机译:雌性恒河猴基底下丘脑中KiSS-1,GPR54和促黄体生成激素释放激素(LHRH-1)mRNA的绝经后增加。

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摘要

The G-protein coupled receptor, GPR54, and its ligand, kisspeptin-54 (a KiSS-1 derived peptide) have been reported to be important players in control of LHRH-1 release. However, the role of the GPR54 signaling in primate reproductive senescence is still unclear. In the present study we investigated whether KiSS-1, GPR54, and LHRH-1 mRNA in the brain change after menopause in female rhesus monkeys using quantitative real-time PCR. Results indicate that KiSS-1, GPR54, and LHRH-1 mRNA levels in the medial basal hypothalamus (MBH) in postmenopausal females (28.3+/-1.1 years of age, n=5) were all significantly higher than that in eugonadal adult females (14.7+/-2.1 years of age, n=9), whereas KiSS-1, GPR54, and LHRH-1 mRNA levels in the preoptic area (POA) did not have any significant changes between the two age groups. To further determine the potential contribution by the absence of ovarian steroids, we compared the changes in KiSS-1, GPR54, and LHRH-1 mRNA levels in young adult ovarian intact vs. young ovariectomized females. Results indicate that KiSS-1 and LHRH-1 mRNA levels in the MBH, not POA, in ovariectomized females were significantly higher than those in ovarian intact females, whereas GPR54 mRNA levels in ovariectomized females had a tendency to be elevated in the MBH, although the values were not quite statistically significant. Collectively, in the primate the reduction in the negative feedback control by ovarian steroids appears to be responsible for the aging changes in kisspeptin-GPR54 signaling and the elevated state of the LHRH-1 neuronal system.
机译:据报道,G蛋白偶联受体GPR54及其配体Kisspeptin-54(一种KiSS-1衍生肽)是控制LHRH-1释放的重要参与者。但是,GPR54信号在灵长类动物生殖衰老中的作用仍不清楚。在本研究中,我们使用定量实时PCR研究了雌性恒河猴绝经后大脑中KiSS-1,GPR54和LHRH-1 mRNA的变化。结果表明,绝经后女性(28.3 +/- 1.1岁,n = 5)的内侧下丘脑(MBH)中的KiSS-1,GPR54和LHRH-1 mRNA水平均显着高于成年雌性成年女性。 (14.7 +/- 2.1岁,n = 9),而在两个年龄组之间,视前区(POA)的KiSS-1,GPR54和LHRH-1 mRNA水平没有明显变化。为了进一步确定缺乏卵巢类固醇的潜在作用,我们比较了卵巢成年女性和未卵巢切除的年轻女性中KiSS-1,GPR54和LHRH-1 mRNA水平的变化。结果表明,去卵巢女性的MBH而非POA中的KiSS-1和LHRH-1 mRNA水平显着高于卵巢完整女性中的KiSS-1和LHRH-1 mRNA水平,而去除卵巢的女性中GPR54 mRNA水平却有升高的趋势,尽管该值在统计上不太显着。总体而言,在灵长类动物中,卵巢类固醇对负反馈控制的减少似乎是导致kisepteptin-GPR54信号的衰老变化和LHRH-1神经元系统状态升高的原因。

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